TNFα protein secreted by MDM cells exposed to over time was measured by ELISA

<p><b>Copyright information:</b></p><p>Taken from "Cytokine responses of bovine macrophages to diverse clinical subspecies strains"</p><p>BMC Microbiology 2006;6():10-10.</p><p>Published online 14 Feb 2006</p><p>PMCID:PMC1382238.</p><p>Copyright © 2006 Janagama et al; licensee BioMed Central Ltd.</p> Total amount of protein (pg/ml) is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of TNFα expression for each strain). Intracellular bacterial numbers were calculated based on the genome size of and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 secreted low amounts of TNFα protein relative to other cell stimulations