Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma-5

<p><b>Copyright information:</b></p><p>Taken from "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/7/126</p><p>BMC Cancer 2007;7():126-126.</p><p>Published online 12 Jul 2007</p><p>PMCID:PMC1940018.</p><p></p>s livers, where β-actin was used as an internal control. The bisulfite-treated DNA sequencing was preformed on the genomic region (-160 ~ + 200) around TSS of SFRP1, where the extent of methylation of the promoter was evaluated through DNA sequencing on 8 random clones inserted by PCR products. There were 56 CpG dinucleotides (CpGs), represented by circles, located on the region. Black and white circles represented the methylated and unmethylated CpG dinucleotides, respectively, where the methylation status was determined by bisulfite-treated DNA sequencing on the corresponding clones. C, HCCs; N, non-cancerous livers