Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma-0

<p><b>Copyright information:</b></p><p>Taken from "Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/7/126</p><p>BMC Cancer 2007;7():126-126.</p><p>Published online 12 Jul 2007</p><p>PMCID:PMC1940018.</p><p></p>nt non-cancerous livers (N), where β-actin was employed as an internal control. Each PCR was generally performed in 32 thermal cycles and PCR products were visualized after electrophoresis through 2% agarose. The length of PCR product of SFRP1 and β-actin are 328 bp and 295 bp, respectively. To confirm the absence of influences of genomic DNA contamination, 6 Paired HCCs and non-HCCs were selected randomly to detect the SFRP1 and beta-actin expression by RT-PCR. Experiments were performed by using RT (RT+) or no RT (RT-) in each sample. Real time RT-PCR analysis of SFRP1 was carried out on 46 paired HCCs and adjacent non-cancerous livers. For each sample, the relative mRNA level of SFRP1 was normalized based on that of β-actin. The line within each box represents the median -ΔCt value; the upper and lower edges of each box represent the 75th and 25th percentile, respectively; the upper and lower bars indicate the highest and lowest values determined, respectively. * indicates p valu