<p>(A) The basic promoter region of the sFRP-4 gene has TATA-box located 38 bases upstream of the transcription start site, around which CpG dinucleotides are clustered (shown in boldface). (B) Twelve independent bisulfite mappings. Corresponding to the repression of the <i>sFRP-4</i> gene expression in steady-state ST2 cells, CpGs within the mouse <i>sFRP-4</i> gene basic promoter, especially upstream of TATA-box, are highly methylated. (C) The effect of 2 nM of 5-aza-dC treatment for 72 hr on steady-state <i>sFRP-4</i> expression. Although not markedly prominent, quantitative RT-PCR showed that the steady-state expression of <i>sFRP-4</i> was, albeit two-fold at most, significantly restored by 5-aza-dC treatment. The statistical significa...
<p><b>Copyright information:</b></p><p>Taken from "Down-regulation of SFRP1 as a putative tumor supp...
<p><b>A</b>) A schematic depiction of the distinct <i>Oxtr</i> promoter/<i>EYFP</i> constructs used;...
CpG methylation is known to suppress transcription. This repression is generally thought to be relat...
<p>A, Schematic diagram of the SFRP-1 promoter region (blue-colored regions: CpG islands). CpG islan...
Tivity is shown as the mean ± SD based on the activity of pGL4 (shown as construct 0) as 1 from thre...
Tivity is shown as the mean ± SD based on the activity of pGL4 (shown as construct 0) as 1 from thre...
<p>A, D) Nucleotide sequences of Jun and Myc promoter region fragments (upper strands) and the corre...
<p><i>In vitro</i> binding of nuclear protein from HeLa cells to TATA-box and CpGs 5 bases upstream ...
<p>A. The CpG islands of mouse <i>Tex13</i> were predicted with the MethPrimer program (<a href="htt...
<p>(A) mRNA expression of SFRP1 at each differentiation stages was analyzed in both mES and P-iPS ce...
To determine if: (1) 5' CpG island methylation is related to Fhit inactivation; (2) there are tumor ...
International audienceBackgroundTransgenes are often engineered using regulatory elements from dista...
<p>From the MeDIP/CGI array-generated methylation profiles, ten cocaine-induced abnormally methylate...
Methylation is the only known modification of DNA and in animals it mainly occurs at cytosines in a ...
<p><b>(A)</b> A schematic representation of the CpG island, predicted in the 5’-regulatory region of...
<p><b>Copyright information:</b></p><p>Taken from "Down-regulation of SFRP1 as a putative tumor supp...
<p><b>A</b>) A schematic depiction of the distinct <i>Oxtr</i> promoter/<i>EYFP</i> constructs used;...
CpG methylation is known to suppress transcription. This repression is generally thought to be relat...
<p>A, Schematic diagram of the SFRP-1 promoter region (blue-colored regions: CpG islands). CpG islan...
Tivity is shown as the mean ± SD based on the activity of pGL4 (shown as construct 0) as 1 from thre...
Tivity is shown as the mean ± SD based on the activity of pGL4 (shown as construct 0) as 1 from thre...
<p>A, D) Nucleotide sequences of Jun and Myc promoter region fragments (upper strands) and the corre...
<p><i>In vitro</i> binding of nuclear protein from HeLa cells to TATA-box and CpGs 5 bases upstream ...
<p>A. The CpG islands of mouse <i>Tex13</i> were predicted with the MethPrimer program (<a href="htt...
<p>(A) mRNA expression of SFRP1 at each differentiation stages was analyzed in both mES and P-iPS ce...
To determine if: (1) 5' CpG island methylation is related to Fhit inactivation; (2) there are tumor ...
International audienceBackgroundTransgenes are often engineered using regulatory elements from dista...
<p>From the MeDIP/CGI array-generated methylation profiles, ten cocaine-induced abnormally methylate...
Methylation is the only known modification of DNA and in animals it mainly occurs at cytosines in a ...
<p><b>(A)</b> A schematic representation of the CpG island, predicted in the 5’-regulatory region of...
<p><b>Copyright information:</b></p><p>Taken from "Down-regulation of SFRP1 as a putative tumor supp...
<p><b>A</b>) A schematic depiction of the distinct <i>Oxtr</i> promoter/<i>EYFP</i> constructs used;...
CpG methylation is known to suppress transcription. This repression is generally thought to be relat...