Immunochemical detection of endogenous XRCC1 and PCNA at DNA repair sites

<p><b>Copyright information:</b></p><p>Taken from "XRCC1 and PCNA are loading platforms with distinct kinetic properties and different capacities to respond to multiple DNA lesions"</p><p>http://www.biomedcentral.com/1471-2199/8/81</p><p>BMC Molecular Biology 2007;8():81-81.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2039748.</p><p></p> Widefield fluorescence images of Hela cells are shown. Cells were fixed at indicated time points after laser microirradiation. (A) Both, XRCC1 and PCNA, accumulate at laser-induced DNA damage sites. (B) Microirradiated Hela cells were extracted with 0,5% Triton-X100 and 500 mM NaCl prior to fixation. After in situ extraction no endogenous XRCC1 can be detected at microirradiated sites while PCNA accumulations can still be observed. Scale bars, 5 μm