Accumulation of XRCC1 and LIGIIIα at SSBs after local UV-irradiation in XPA-UVDE cells

<p><b>Copyright information:</b></p><p>Taken from "Translocation of XRCC1 and DNA ligase IIIα from centrosomes to chromosomes in response to DNA damage in mitotic human cells"</p><p>Nucleic Acids Research 2005;33(1):422-429.</p><p>Published online 14 Jan 2005</p><p>PMCID:PMC546168.</p><p>© 2005, the authors © </p> Co-localization of XRCC1 with LIGIIIα was identified by double immunolabeling. Two minutes after local UV-irradiation (20 J/m) cells were fixed with paraformaldehyde and co-stained with anti-XRCC1 antibody (red, upper row) and anti-LIGIIIα antibody (green, middle row); the column c is for XPA-UVDE cells treated with DIQ, an inhibitor of PARP, before UV-irradiation. Co-localization of XRCC1 with LIGIIIα appears yellow in overlay (bottom row)