Recruitment of BER factors to sites of laser-induced DNA damage in Ku70-deficient cells.

<p>Recruitment of XRCC1 and PAR polymer accumulation were measured at sites of DNA damage in Ku70-deficient cells as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118819#sec002" target="_blank">Materials and Methods</a>. (A) Images obtained one minute after irradiation of Ku70-deficient cells with and without 150 μM BPA. (B) Time courses of XRCC1 recruitment and PAR synthesis to the site of damage in Ku70-deficient cells with the repair times specified. Images are representative; at least 15 cells were measured for every time point and each bar represents mean ± SEM of three independent experiments (*p < 0.05 vs. control). Scale bars are 20 μM.