Recruitment of XPC to DNA damage occurs in the absence of centrin.

<div><p><b>A</b>. Immunofluorescence micrograph shows the recruitment of GFP-cXPC to laser-induced DNA damage. Antibodies to cyclopyrimidine dimers were used to visualise the damaged region (red), which colocalises with GFP-cXPC (green). DNA was labelled with DAPI (blue). Scale bar, 5 µm.</p> <p><b>B</b>. Live-cell visualisation of GFP-cXPC recruitment to DNA damage in cells of the indicated genotype. Micrographs show the GFP channel at different times after irradiation. Different GFP-cXPC expression levels were examined for recruitment kinetics. Scale bar, 5 µm.</p> <p><b>C</b>. Quantitation of GFP-cXPC recruitment to the stripe of laser-induced DNA damage. Curve shows the mean increase over background <u>+</u> s.d. for measurements taken in wild-type (<i>N</i>=36), centrin-deficient (<i>N</i> =34) and rescued (<i>N</i> =31) cells.</p>