Confocal immunofluorescent microscopy images of F1- and V-antigen expression for X85MF1 and X85V bacterial strains within mouse macrophage-like J774 A1 cells

<p><b>Copyright information:</b></p><p>Taken from "A comparison of immunogenicity and protective immunity against experimental plague by intranasal and/or combined with oral immunization of mice with attenuated serovar Typhimurium expressing secreted F1 and V antigen"</p><p></p><p>Fems Immunology and Medical Microbiology 2007;51(1):58-69.</p><p>Published online 19 Jul 2007</p><p>PMCID:PMC2121146.</p><p>© 2007 Wen-Tssann Liu Journal compilation © 2007 Federation of European Microbiological Societies</p> The J774 A.1 cells were infected with the vaccine strain, and the intracellular bacteria were probed with anti-F1 or anti-V mAb and then detected with Alex-Red conjugated anti-mouse IgG (red). The cell morphology, as characterized by the cytoskeleton, was observed by Phllodin-FITC-conjugated antitubulin antibody (green)