Immunolocalization of bacteria in mice infected with BC7 mutants or BC7 <i>cblS</i> complement.

<p>Mice were infected with the BC7 <i>adhA</i>, BC7 <i>cblA</i>, or BC7 <i>cblS</i> mutants or the BC7 <i>cblS</i> complement suspended in alginate and sacrificed at 1 d post-infection. Paraffin lung sections from these mice were deparaffinized and incubated with antibody to <i>B. cenocepacia</i>. Bound antibody was detected by second antibody conjugated with AlexaFluor 488 and sections were counterstained with hematoxylin. Sections were viewed under fluorescence microscope. A, C, E and G shows bacteria in lung sections from mice infected with BC7 <i>adhA</i>, BC7 <i>cblA</i>, BC7 <i>cblS</i>, and the BC7 <i>cblS</i> complement, respectively. B, D, F and H are bright field images of A, C, E and G respectively. Arrows point to bacteria in the lung. Images are representative of three mice per group.