CBA/Ca mice display uncontrolled lung inflammation and associated apoptosis following <i>S. pneumoniae</i> infection.

<p>All results are from intranasal-infection of mice with wild-type <i>S. pneumoniae</i> D39. (A) Number of IFNγ⁺NKp46⁺CD4⁻ cells per mg lung in PBS-treated or <i>S. pneumoniae</i>-infected BALB/c and CBA/Ca mice at 24 hours p.i. (B) Top left shows gating of NK cells in lung homogenate. Remaining panels show example IFNγ-staining from PBS-treated CBA/Ca or <i>S. pneumoniae</i>-infected BALB/c and CBA/Ca mice at 24 hours p.i. (C) Proportion (%) of apoptotic cells in areas of inflammation within lungs of <i>S. pneumoniae</i>-infected BALB/c and CBA/Ca mice. Inset shows apoptotic cell (arrow) within area of inflammation as defined by presence of inflammatory infiltrate. White bars=BALB/c, black bars=CBA/Ca. *'s indicate significant difference, where *=p<0.05 and ***=p<0.005. For all graphs data represent mean +/− SEM. Results in (A and B) are representative of 3 independent experiments with >4 mice per group. Results in (C) are from a single experiment with 5 mice per group. PBS-treated groups contained both BALB/c and CBA/Ca mice.