MOESM6 of Development of safe, effective and immunogenic vaccine candidate for diarrheagenic Escherichia coli main pathotypes in a mouse model

Additional file 6: Figure S6. Evaluation of IFNγ levels elicited by unadjuvanted combined vaccine candidate. Balb/C mice (n = 10 mice per group) were immunized subcutaneously with 109 CFU of formalin killed whole cell antigens. Antigens belonged to the above-mentioned five-diarrheagenic E. coli pathotypes. Combined vaccine candidate consisted of formalin-killed whole cell of the main five pathotypes. Post-immunization blood samples were collected from mice groups weekly for six weeks. At week seven, mice were challenged with 106 CFU intraperitoneally and blood samples were collected one week after the challenge. The concentration of IFNγ was measured for all seven intervals. IFNγ concentration of combined vaccine candidate at selected time points compared to A) EAEC antigens, B) EPEC antigens, C) EIEC antigens, D) EHEC antigens and E) ETEC antigens. *p < 0.05, ** p < 0.001, and ***p < 0.0001, each bar represents mean ± standard deviation