Calretinin, calbindin-28 kDa, and NF-200 kDa immunofluorescence in the ganglion cell layer (GCL)/nerve fiber layer (NFL) as seen in retinal whole-mounts

Double labeling for calretinin (red) and NF-200 kDa (green) shows retinal ganglion cell (RGC) somata, surrounded peripherally by light NF-200 kDa immunofluorescence (arrows), that were not immunopositive for calretinin. Calretinin immunofluorescence was present at discrete locations intermittently along the long axis of the RGC nerve fiber, whereas the NF-200 kDa immunofluorescence was uniform. Channel intensity profiles for the red and green channels along the long axis (lines a and b in shown in and respectively) revealed that for regions on the long axis where staining for calretinin was prominent, staining for NF-200 kDa was less prominent () and vice versa (). Double labeling for calbindin-28 kDa (red) and NF-200 kDa (green) showed that calbindin-28 kDa-positive immunofluorescence was smoothly distributed in the nerve fibers similar to NF-200 kDa. RGC somata that were surrounded peripherally by light NF-200 kDa immunofluorescence (arrow) were also stained with calbindin-28 kDa while for others (arrowhead) staining was less prominent. Channel intensity profiles for the red and green channels for straight lines along the long axis (lines d and e in shown in and J respectively) presented some region where immunofluorescence for NF-200 kDa was prominent while that for calbindin-28 kDa was less prominent () and others where the intensity profiles were similar (). Scale bar represents 20 µm.Abbreviations: bv is blood vessel.<p><b>Copyright information:</b></p><p>Taken from "Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina"</p><p></p><p> 2008;14():1600-1613.</p><p>Published online 31 Aug 2008</p><p>PMCID:PMC2528027.</p><p>