Calretinin and calbindin-28 kDa are distinctly distributed in the ganglion cell and nerve fiber layer as seen in retinal whole-mounts

Double labeling for calretinin (red) and calbindin-28 kDa (green) in the ganglion cell layer (GCL)/nerve fiber layer (NFL) shows that labeling for each was present in a distinct set of neurons. Calbindin-28 kDa positive cell bodies are indicated by arrowheads. Some calretinin-positive neurons show processes (arrow) that ascend distally. Note the discontinuous staining pattern of calretinin in the NFL in contrast to a smoother staining pattern for calbindin-28 kDa. , A single confocal optical section distal to that of shows that the calretinin positive processes (arrow) are directed in the inner plexiform layer (IPL) distally toward a calretinin-immunopositive plexus, a characteristic of displaced amacrine cells. Representative calretinin and calbindin-28 kDa double staining in the GCL/NFL is shown. Channel intensity profiles for the red and green channels for straight lines along the long axis (lines a and b in shown in and respectively) show different intensity profiles for calretinin and calbindin-28kDa immunofluorescence. Scale bar represents 20 µm.<p><b>Copyright information:</b></p><p>Taken from "Subcellular compartmentalization of two calcium binding proteins, calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina"</p><p></p><p> 2008;14():1600-1613.</p><p>Published online 31 Aug 2008</p><p>PMCID:PMC2528027.</p><p>