Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mice-6

Ssion. TA muscles were given 10 twice-weekly intramuscular injections of 5 μg of ESO complexed with the NG-PEI2K(PEG550)copolymer, and harvested 6 wks after the first injection. () Images of serial whole transverse sections immunolabeled for dystrophin (A) and nNOS (B) show the correlation in ESO-mediated upregulation of these two membrane-associated proteins. Higher magnification images of specific regions from panels A and B show clearly the concomitant (co-localized) upregulation of both dystrophin and nNOS in the same set of fibers of the polyplex-injected muscles. As expected nNOS expression in untreated controls was negligible.<p><b>Copyright information:</b></p><p>Taken from "Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mice"</p><p>http://www.biomedcentral.com/1472-6750/8/35</p><p>BMC Biotechnology 2008;8():35-35.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2362111.</p><p>