12 h after CLP or sham laparotomy, the numbers of viable splenocytes (A) and infiltrating peritoneal lymphocytes (B) were counted on a hemocytometer (BrightLine; Hausser Scientific) after Trypan blue staining (three to five mice per group)

Peritoneal cells are expressed per milliliter of peritoneal fluid. (C) Peritoneal cells pooled from three to five mice 12 h after CLP were cultured without restimulation for 24 h, and the supernatant cytokine levels were determined with a cytometric bead array. Peritoneal cells from mice that underwent sham laparotomy secreted a minimal amount of cytokines (not depicted). Data shown are means of values ± SEM obtained from individual mice (except for cells pooled in C) and are representative of at least two independent experiments. *, P < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1277-1283.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413026.</p><p>