Depletion of Tregs did not alter cell migration into the peritoneum.

<p>DEREG and C57BL/6 mice pre-treated with DT (1 µg in 100 µl PBS i.v. on days −2 and −1) were subjected to CLP (n = 7–9 mice/group) or left untreated (n = 5 mice/group). Cells from the peritoneal cavity were harvested by peritoneal lavage 24 h after CLP. The total number of cells was enumerated and related to volume of recovered lavage fluid (A). The numbers of B cells (CD19⁺) (B), neutrophils (Ly6G⁺) (C), peritoneal macrophages (F4/80⁺CD11b⁺MHCII⁺) (D) and inflammatory monocytes (F4/80^-CD11b⁺MHCII^lo) (E) was assessed via flow cytometry. Means are shown.