Peritoneal inflammation 48 hrs after infection.

<p>Chemokines (MCP1, Mip1α, Rantes) were determined in the peritoneal lavage fluid of mice (n = 6) after 48 hrs of infection (6A). Peritoneal exudate cells were isolated 48 h after i.p. infection with 1×10∧6 Lm, adherent cells were enriched and RNA prepared and subjected to qPCR for IL12p40, IL6 and IL10 (6B). Data from 3–5 experiments (n = 10–15) are pooled and means with standard deviation are shown. 48 h after i.p infection peritoneal exudate cells were isolated and cytospins stained with Wright-Giemsa solution. The amount of cells (6C), the percentage of macrophages (6D) and the percentage of neutrophils (6E) were counted and depicted (n = 9).