(A) MPO activity in the lungs, heart, liver, kidney, and intestine of WT and TG mice treated with saline or LPS for 4 h

Compared with WT-Con and TG-Con mice, WT-LPS mice showed a marked increase in MPO activity in all five organs, which was significantly reduced in TG-LPS mice. The means ± SEM of seven animals are shown. *, P < 0.05 compared with the other three groups. (B) Neutrophil counts in BAL fluids. Compared with WT-Con and TG-Con mice, WT-LPS mice had a marked increase in BAL fluid neutrophil count, which was reversed in TG-LPS mice. The means ± SEM of seven animals are shown. *, P < 0.05 compared with the other three groups. (C) Histopathological evaluation of PMN infiltration and organ injury. Paraffin-embedded sections were prepared from the lungs, kidney, and liver of WT and TG mice treated with saline or LPS for 5 h, and subjected to hematoxylin and eosin staining. Lungs of WT-Con and TG-Con mice show thin alveolar wall and normal cellularity (C1 and C2). The lung of a WT-LPS mouse shows intense cellular infiltration, alveolar septal wall thickness, interstitial edema, and alveolar congestion (C3). Kidneys of WT-Con and TG-Con mice show normal histological structures of cortex with glomerulus (C5 and C6). The kidney of a WT-LPS mouse shows increased cellular infiltration, glomerular and sinusoidal congestion, and signs of tubular swelling and cell injury (C7). Livers of WT-Con and TG-Con mice show a normal histological appearance of the central vein surrounded by hepatocytes and sinusoids (C9 and C10). The liver of a WT-LPS mouse shows a congested central vein, hemorrhage, and signs of hepatic cell injury and necrosis (C11). The pathological changes caused by LPS were significantly alleviated in TG-LPS mice in all three organs (C4, C8, and C12). Bars, 100 μm.<p><b>Copyright information:</b></p><p>Taken from "Divergent roles of endothelial NF-κB in multiple organ injury and bacterial clearance in mouse models of sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1303-1315.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413029.</p><p>