Fibroblasts prespread on a recombinant integrin ligand (50K) were stimulated with heparin-binding fragments of fibronectin (H/0) or a heparin-binding mutant (H/0-GAG) before fixation and staining for p190-A (green) and the lipid raft marker GM-1 (red)

(A) Immunostaining of p190-A and GM-1, including masks used to measure the pixel intensity of the lamellar border. Bar, 5 μm. (B–E) Pixel intensity of p190-A (B and D) and GM-1 (C and E) within a 0.5-μm-thick border around the edge of each lamella after stimulation with H/0 (B and C) or H/0-GAG (D and E). The pixel intensity of 20 different cells was measured for each time point. The experiment was repeated on six separate occasions. Error bars represent standard error. *, significant change (P < 10).<p><b>Copyright information:</b></p><p>Taken from "p190RhoGAP is the convergence point of adhesion signals from αβ integrin and syndecan-4"</p><p></p><p>The Journal of Cell Biology 2008;181(6):1013-1026.</p><p>Published online 16 Jun 2008</p><p>PMCID:PMC2426943.</p><p>