MEFs prespread on a recombinant integrin ligand (50K) were stimulated with heparin-binding fragments of fibronectin (H/0) before measuring the RhoA activity of lysates by ELISA

Wild-type MEFs (A), p190-A–null MEFs (B), p190-A–null MEFs reexpressing p190-A (C), MEFs transfected with a nontargeting siRNA (E), and MEFs transfected with an siRNA targeted against p190-A (F) are shown. (G) Direct comparison of RhoA activity in cells fully spread on 50K after transfection with a nontargeting or p190-A–targeted siRNA. (D and H) Expression levels of p190-A in null and reexpressing MEFs (D) or MEFs transfected with siRNAs (H); lysates were also probed for the related p190-B isoform to eliminate the possibility of molecular compensation or off-target knockdown. Equal loading between time points was confirmed by a total protein assay. Error bars represent standard error. *, significant change (P < 0.05) from experiments repeated on at least four separate occasions.<p><b>Copyright information:</b></p><p>Taken from "p190RhoGAP is the convergence point of adhesion signals from αβ integrin and syndecan-4"</p><p></p><p>The Journal of Cell Biology 2008;181(6):1013-1026.</p><p>Published online 16 Jun 2008</p><p>PMCID:PMC2426943.</p><p>