Cell adhesion and spreading.

<p>A. Adhesion of HEK293T transfectants to surfaces coated with 20 µg/mL fibrinogen. The amount of bound cells was determined by measuring LDH activity as described in Materials and Methods. Data are representative of three independent experiments, each in triplicate. Error bars are SD. B. Quantification of the areas of adhering/spreading cells as described in Materials and Methods. C. DIC images of HEK293T transfectants after adhering to immobilized fibrinogen at 37°C. a: WT; b: A252D; c: A252D/D126A; d: A252D/D127A; e: A252D/D126A/D127A. The images are representatives of three independent experiments.