Cell adhesion of DDR-overexpressing HEK293 cells to collagen I or collagen-derived peptides.

<p>(A) Time course of cell adhesion to collagen I. Cell adhesion assays were carried out in 96-well plates with fluorescently labeled cells. 293-DDR cells or empty-vector control cells (293-EV) were allowed to adhere to collagen I-coated wells for the indicated duration at 37°C. Cell adhesion was measured and calculated as described in Materials and Methods. Data shown are representative of four independent experiments, each performed in triplicate. (B) Cell adhesion to collagen I, integrin-specific peptide (GLOGER) or DDR-specific peptide GPRGQOGVNleGFO (GVMGFO). Cells were allowed to adhere to different substrates for 1 h at 37°C in the presence or absence of 10 µg/ml β1-integrin blocking mAb P5D2. The data are representative of at least four independent experiments, each performed in triplicate. The error bars indicate the sample standard deviation.