Endothelial-specific ablation of Serum Response Factor causes hemorrhaging, yolk sac vascular failure, and embryonic lethality-8

of wild-type (A) and (B) whole yolk sac at E13.5; arrowheads (B) mark apparent blood vessel remnants in mutant yolk sac tissue. Photomicrographs (200×) of cross-sections of wild-type (C) and (D and E) yolk sac at E13.5 immunolabelled for PECAM-1 (brown-gray = PECAM-1, red = neutral red counterstain). Normal cellular layers of endoderm (Ed) and extraembryonic mesoderm (Md) are tightly associated in wild-type as compared to mutant yolk sac tissue. Asterisks mark areas of inappropriate tissue layer separation in yolk sac (D). (E) Quantitation of laminar separation in cross-sections of yolk sac tissues as in C and D revealed increased delamination in mutant animals (16.3 ± 10.8% vs. 62.0 ± 10.4%, wild-type vs. mutant respectively; p = 0.0031). Scale bar: C, D = 50 μm.<p><b>Copyright information:</b></p><p>Taken from "Endothelial-specific ablation of Serum Response Factor causes hemorrhaging, yolk sac vascular failure, and embryonic lethality"</p><p>http://www.biomedcentral.com/1471-213X/8/65</p><p>BMC Developmental Biology 2008;8():65-65.</p><p>Published online 20 Jun 2008</p><p>PMCID:PMC2442838.</p><p>