Schematic of the transgene.

<p>A. Schematic of the transgene showing the four regions analyzed for histone modifications, along with their base-pair position relative to the 5′ end of the transgene. We determined enrichment of H3K4me2 (B), H3K9me3 (C), H3K9me2 (D), and H3K27me3 (E) in four Tg5S lines (black circles) and two control Tg0 lines (white circles). Each heatmap illustrates the relative enrichment of that modification at each position in each line. For comparison, heatmaps of <i>Tk</i> mRNA levels, nucleolar localization, TFIIIC65 enrichment, and copy number for each line are shown in (F). ND, not determined.