Comparision of histone modifications across a normal and deleted version of the same X centromere.

<p>The schematics at the top show the structure of the normal <i>(A)</i> and partially deleted <i>(B)</i> X centromere. Each chromosome is contained in a human-hamster cell line. The numbers along the centromere represents genomic sites interrogated by ChIP-PCR with a specific histone antibody. The line plots shows average relative enrichment for each histone modification across the centromere region (n = 3 with SD). Control regions, including GAPDH and and X-linked ZXDA are also included. The bar graph shows relative enrichment for H3K27 methylation (n = 3 with SD) calculated as percentage of input. Asterisks indicate statistically significant differences in enrichment for a modification at a particular site between the normal X and deleted X as determined by a Student's t-test (<i>p</i><0.05).