<i>pudgy</i> mutants have an altered lipid catabolic profile upon fasting.

<p>(A) <i>pdgy[BG]</i> mutants have significantly improved survival under starvation conditions. Control w¹¹¹⁸ (dashed line) and <i>pdgy[BG]</i> (solid line) 3-day-old males starved on 0.8% agarose/PBS (n = 90, log rank P = 3×10⁻⁸). (B–B′) <i>pdgy[BG]</i> mutants display delayed lipid catabolism upon starvation. Relative total body triglycerides normalized to total body protein of control and <i>pdgy[BG]</i> L2 larvae (84 h AEL) (B) or adults (B′) fasted for 0, 2, 4, 6 or 8 hours. (C) Nile red staining of early L3 larval fat bodies reveals larger lipid droplets in <i>pdgy[BG]</i> mutants compared to controls, both under fed conditions and when starved for 24 h on 0.8% agarose/PBS. Scare bars: 50 µm. (D) <i>Pudgy</i> mutants have aberrant levels of free fatty acids. Free fatty acid levels obtained by lipidomic profiling of control and <i>pdgy[BG]</i> mutant adults under fed and 16-hour fasting (“starved”) conditions. Measurements annotated with different letters are significantly different from each other (ttest<0.01). # indicates lipid species for which the drop in concentration observed in control animals upon fasting is significantly impaired in the mutant. (E–E′) <i>pdgy[BG]</i> mutants have an altered profile of lipid catabolism upon fasting. Levels of TAG(39∶1) (E) and TAG(53∶3) (E′) were quantified by UPLC-MS lipidomic profiling of control or <i>pdgy[BG]</i> mutant adult males after 0 and 16 hours of fasting. For all panels, assays done in triplicate, Error bars: Std. Dev., * ttest<0.05, ** ttest≤0.01, ***ttest<0.001.