Sugar sensitivity of <i>FASN</i> null mutants.

<p>(A) Survival until metamorphosis or early larval lethality (<b>†</b>) of <i>w</i>^<i>-</i> control (Co) and <i>FASN</i>^{<i>Δ24-23</i>} mutant (<i>Δ24-23</i>) animals fed either a LCD (Lipids -) or a beySD (Lipids +) with (10%) or without (0%) additional sugar. For each condition, the mean of survival rate was calculated by assessing the number of newly hatched larvae reaching metamorphosis (groups of 100 L1 larvae placed in 5 separate tubes). (B-D) Metabolic measurements from <i>w</i>^<i>-</i> control (Co) or <i>FASN</i>^{<i>Δ24-23</i>} mutant (<i>Δ24-23</i>) larvae that were fed a beySD until L2/L3 transition and then transferred onto fresh media (0%) or media containing additional sucrose (10%) for 24h. (B) Blue dye accumulation in 24h old L3 larvae after feeding on a tinted media for 1h (means calculated from 3 samples of 10 feeding L3 larvae); experiments repeated 3 times. (C-D) Circulating glucose (C) and trehalose (D) levels from bled larvae (means calculated from 4 samples of 20 to 30 bled L3 larvae); experiment repeated 3 times. (E-L) Membrane localization of tGPH in FB explants incubated with (F,H,J,L) or without (E,G,I,K) insulin (0,5 μM) (for quantification of tGPH intensity see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004995#pgen.1004995.s004" target="_blank">S4B Fig</a>.). FB explants were dissected from <i>w</i>^<i>-</i> control (E,F,I,J) or <i>FASN</i>^{<i>Δ24-23</i>} (G,H,K,L) L3 larvae that were fed a beySD (E-H) or the same media supplemented with 10% sucrose (I-L) for 24h. For each genotype, at least 10 larvae were dissected; experiment repeated twice. Scale bars: 20μm.