Genetic screen for Rabs that affect the size of lipid droplets.

<p>(A) Nile red staining of lipid droplets in wandering stage third instar larval fat body cells. Dominant-negative (DN) Rab expression reduces (DN-Rab1 and DN-Rab5) or increases (DN-Rab7 and DN-Rab10) lipid droplet size. Expressing DN-Rab18 does not affect the size of lipid droplets. Scale bar: 10 µm. (B) Quantification of the effects of all DN-Rabs. The error bars represent the standard deviation. Red dashed lines indicate the average variation in lipid droplet size in controls. *: P<0.01. (C) Nile red staining of lipid droplets in wandering stage third instar larval fat body cells. Constitutive-active (CA) Rab expression reduces (CA-Rab39) or increases (CA-Rab1, CA-Rab10, and CA-RabX4) lipid droplet size. Expressing CA-Rab18 does not affect the size of lipid droplets. <i>bmm</i> mutant was included as a comparison. Scale bar: 10 µm. (D) Quantification of the effects of all CA-Rabs. The error bars represent the standard deviation. Red dashed lines indicate the average variation in lipid droplet size in controls. *: P<0.01.