Increased lipid droplet aggregates were observed in CGI-58-deficient Dauers.

<p><b>(A)-(F)</b><i>cgi-58</i>, but not <i>atgl-1</i>, limits the formation of lipid droplet aggregates in both control and AMPK mutant dauer larvae. Dauer larvae were stained with C₁-BODIPY-C₁₂ 32 hours after being shifted to 25°C. Lipid droplets were subsequently isolated from the stained dauer larvae. Scale bar = 10 μm. All strains carry <i>daf-2(e1370)</i> in <b>(A)-(F) and (I)</b>. <b>(G)-(H)</b> <i>CGI-58</i> limits the formation of lipid droplet aggregates in MEFs. MEFs were stained with C₁-BODIPY-C₁₂ and imaged using a 100x objective. Scale bar = 4 μm. <b>(I)</b> Quantification of the percentage of lipid droplet aggregates per frame (for dauer larvae) or per cell (for MEFs) described in <b>(A)-(H)</b> determined for 1000 lipid droplets/100 cells analyzed for each respective genotype. Lipid droplets with a volume greater than 20 μm³ are considered as aggregates. ** indicates statistical significance (P<0.01) to all other columns.