<p>Summary of time constants of deactivation for all mutants at <i>V</i> = <i>V</i><sub>0.5</sub>−40 mV (A) and at <i>V</i> = <i>V</i><sub>0.5</sub>−130 mV (B). Filled bars indicate values that are statistically significantly different to WT (* p<0.05, *** p<0.001). † indicates the mutants where values are estimated values based on extrapolation more than 10 mV from the last measured data point (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031640#pone.0031640.s002" target="_blank">Fig. S2</a>).</p
<p>A. Typical currents recorded at 37°C during a protocol to measure rates of deactivation (voltage ...
<p><i>A, B,</i> Normalized current traces recorded during depolarizing voltage steps from −80 to +30...
<p>A) Best fit to AKv1(E2x) channel data is shown in black. Prediction for the steady state inactiva...
<p>Summary of time constants of activation for all mutants at A, <i>V</i> = <i>V</i><sub>0.5</sub>+4...
<p>(A) Current traces correspond to dotted box in the voltage protocol used to measure the recovery ...
<p>A. Typical examples of families of current traces recorded from (i) WT and (ii) Y542A channels du...
<p>A: Envelope test during deactivation at −40 mV. After current activation at −130 mV, pulses to −4...
<p>A. Three state transition model, which includes a low voltage sensitivity step (C1 – C2) and a hi...
<p>A) Isochronal one second inactivation relationship “i”. Mean data points (n = 13) fit to a single...
<p>The energy perturbation caused by mutations for steady-state activation, rates of activation and ...
<p>(A) Family of current traces recorded during a 3 s isochronal activation protocol for (i) WT and ...
(A) shows cell-attached patch currents comparing the inactivation rates of E2117A, E2117D and wild-t...
<p>A) Representative current traces elicited by a 1-s light pulse with a ND = 0 to compare activatio...
Each line represents an average of n≥4 samples. See Fig 3L for growth curves of the mutants that sho...
<p><i>A</i>, <i>B,</i> Normalized current traces for Ca<sub>v</sub>3.1 (thick gray line), Ca<sub>v</...
<p>A. Typical currents recorded at 37°C during a protocol to measure rates of deactivation (voltage ...
<p><i>A, B,</i> Normalized current traces recorded during depolarizing voltage steps from −80 to +30...
<p>A) Best fit to AKv1(E2x) channel data is shown in black. Prediction for the steady state inactiva...
<p>Summary of time constants of activation for all mutants at A, <i>V</i> = <i>V</i><sub>0.5</sub>+4...
<p>(A) Current traces correspond to dotted box in the voltage protocol used to measure the recovery ...
<p>A. Typical examples of families of current traces recorded from (i) WT and (ii) Y542A channels du...
<p>A: Envelope test during deactivation at −40 mV. After current activation at −130 mV, pulses to −4...
<p>A. Three state transition model, which includes a low voltage sensitivity step (C1 – C2) and a hi...
<p>A) Isochronal one second inactivation relationship “i”. Mean data points (n = 13) fit to a single...
<p>The energy perturbation caused by mutations for steady-state activation, rates of activation and ...
<p>(A) Family of current traces recorded during a 3 s isochronal activation protocol for (i) WT and ...
(A) shows cell-attached patch currents comparing the inactivation rates of E2117A, E2117D and wild-t...
<p>A) Representative current traces elicited by a 1-s light pulse with a ND = 0 to compare activatio...
Each line represents an average of n≥4 samples. See Fig 3L for growth curves of the mutants that sho...
<p><i>A</i>, <i>B,</i> Normalized current traces for Ca<sub>v</sub>3.1 (thick gray line), Ca<sub>v</...
<p>A. Typical currents recorded at 37°C during a protocol to measure rates of deactivation (voltage ...
<p><i>A, B,</i> Normalized current traces recorded during depolarizing voltage steps from −80 to +30...
<p>A) Best fit to AKv1(E2x) channel data is shown in black. Prediction for the steady state inactiva...