Gating Phenotype of AAA mutant.

<p>(A) Family of current traces recorded during a 3 s isochronal activation protocol for (i) WT and (ii) AAA mutant channels. (iii) Isochronal activation curves for WT (filled black circle) and AAA mutant (filled grey circle). The mean V_0.5 of isochronal activation for the AAA mutant and WT were −15.1±1.0 mV (n = 4) and −23.1±0.4 mV (n = 4), respectively; see Table S1 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077032#pone.0077032.s001" target="_blank">File S1</a>. (B) Families of −70 mV tail current traces recorded during a 3 s isochronal deactivation protocol for (i) WT and (ii) AAA mutant. The dotted box in the voltage protocol indicates the portion of the traces shown in the current recordings. (iii) Isochronal deactivation curves for WT (filled black circle) and AAA mutant (filled grey circle). Data are presented as mean ± SEM for n = 4 experiments. The mean V_0.5 of isochronal deactivation for the AAA mutant and WT were −23.4±1.1 mV (n = 4) and −61.3±0.8 mV (n = 4), respectively. (C) Typical family of current traces recorded between −60 to −160 mV at 20 mV intervals, corresponding to the dotted box in the voltage protocol, used to measure rates of deactivation for (i) WT and (ii) AAA mutant. (iii) Summary of the rates of deactivation for AAA mutant (grey) and WT (black). Data shown as mean ± SEM (n = 4), error bars are within the symbols.