Analysis of the immune response generated in local draining lymph nodes (LNs) to the site of immunization.

<p>Groups of four mice were immunized as indicated in the bar charts with 10⁷ pfu of MVAwt (white bars) or MVAΔC12L (black bars) and seven dpi specific T-cell responses against the indicated peptides were evaluated in the regional draining LNs to the different immunization routes as depicted in the Figure. The magnitude of the response was measured by IFN-γ Elispot assay after 24 hr stimulation. Background (RPMI control) subtracted data are depicted as mean IFN-γ spot forming units (SFU) per 10⁶ cells ± SD. SC: subcutaneous; IN: intranasal; IM: intramuscular; ILN: inguinal lymph nodes; CLN: cervical lymph nodes. Statistically significant differences: *p<0.05, **p<0.01, ***p<0.001.