ELISpot response following immunization with LLO or LLO peptide.

<p><b>A–B.</b> C57BL/6 mice were immunized in the footpad with 250 picomoles of either LLOWT, LLOWW, or LLO(190–201) peptide emulsified in incomplete Freund's adjuvant. After 7 days, draining lymph nodes were isolated and the number of IFNγ (<b>A</b>) and IL-2 (<b>B</b>) producing cells was determined by ELISpot. Recall antigen was either LLOWW at 10 nM or no antigen. Bars represent the mean+/−SEM for 2–6 mice per group over three independent experiments. Analysis of the data using D'Agostino & Pearson omnibus normality test followed by student t test revealed that the increase in IFNγ production following immunization with LLOWW was significant when compared to both LLOWT or LLO(190–201) immunization (p = 0.0012 for LLOWT vs. LLOWW and p<0.0001 for LLOWW vs. LLO(190–201).