Pax6 expressing FlpIn-3T3 cells have higher proliferation and higher migration capacity compared to FlpIn-3T3 control cells and Pax6(5a) expressing cells.

<p>Control, Pax6 and Pax6(5a) cells were seeded in xCELLigence (Roche) proliferation plates, cells were left on the bench for 1 hr before the plates were put in the xCELLigence machine for measurements for 24 hrs. The measured values were used to calculate the (A) adhesion slope, (B) proliferation slope and (C) doubling time. For adhesion and proliferation slope analyses the mean and standard deviation of three independent experiments are shown. The doubling time analysis shows one representative experiment out of three, because absolute values were changing from experiment to experiment probably due to fluctuations in cell condition. However, described pattern of increased proliferation (Pax6>Pax6(5a)>control) was consistent in all experiments. Each experiment was done in triplicates (A–C). xCELLigence migration plates were used to measure the migration, and to calculate the migration slope (D). One representative experiment out of 3 is shown.