Proliferation and migration in plasma treated fibroblast-like cells.

<p>HSCs were labelled with 25 µM CFSE and incubated with NAC or 5 µg/ml cytochalasin B. Cells were then treated with plasma and incubated for 72 hrs. Panel <b>A</b> outlines representative FACS analysis of cellular proliferation assessed by evaluating the partition of CFSE. Similar results were obtained in nine independent experiments, each performed in triplicate. Panel <b>B</b> reports the percentage of proliferating fluorescent cells. Panel <b>C</b> reports the fluorescence relative to 6-CFDA in cells migrating to the bottom side of trans-well inserts (a.u. means arbitrary unit). Data are reported as mean±SE of results collected in nine independent experiments, each performed in triplicate. ° denotes P<0.02 <i>vs</i> non treated cells. * denotes P<0.05 <i>vs</i> not treated cells. Plasma treated ISEMFs were seeded onto glass coverslips. The cell monolayer was wounded using a plastic tip and 72 hrs later the cells were stained with haematoxylin and eosin. Images were observed and captured using a light transmission microscope connected to a camera (DMLB Leica, Panel <b>D</b>). Similar results were obtained in three independent experiments.