Comparison of parental bipotent cells and MPCs for invasion, migration, anchorage independence and 3D proliferation in matrigel.

<p>(<b>A</b>) Migration assay using transwell chambers were performed on exponentially proliferating indicated cells. Shown here is a bar diagram of the number of cells that migrated in both cell types. The data represents the mean⁺/₋ standard deviation (SD) of three experiments done in six replicates. (<b>B</b>) Invasion assay were performed with indicated cells using matrigel coated transwell chambers. Shown here is a bar diagram of number of cells that invaded in both cell types. The data represent mean⁺/₋ SD of three experiments done in six replicates. (<b>C</b>) 3D matrigel culture. Shown here are phase contrast pictures at day12 using an inverted microscope. (<b>D</b>) Exponentially proliferating cells were analyzed for anchorage independence by soft agar assay. Shown here are images of colonies after 30 days, using inverted microscope.