Cellular localization of wt IRF5 and phosphorylation mutants.

<p>Fluorescence imaging was used to evaluate cellular localization of wt GFP-IRF5 or point mutants in GFP-IRF5 expressed in HT1080 cells. Effects of amino acid substitutions with alanine (A) or aspartic acid (D) are shown in absence or presence of export inhibitor leptomycin B (LMB). *Asterisk notes significant gain of nuclear accumulation with S158D and SS451,462DD. Images represent random sampling of 12 to 15 fields and reflect more than 80% of the population in three independent experiments.