Effect of TbORC1/CDC6, TbORC4, Tb7980 and Tb3120 RNAi on procyclic form <i>T. brucei</i>.

<p><b>A.</b> Analysis of nuclear (N) and kinetoplast (K) DNA configurations in procyclic form <i>T. brucei</i> cells 4 and 6 days post-RNAi induction (induced by tetracycline; Tet+) against <i>TbORC1/CDC6</i>, <i>TbORC4 (13380)</i>, <i>Tb7980 and Tb3120</i>; for each gene, N and K configurations are also shown in cells without RNAi induction (Tet−). Graphs depict the proportion of cells (derived by counting >200 DAPI-stained cells) with conventional 1N1K, 1N2K, or 2N2K configurations, or with any aberrant configuration (others). In each graph, the insert shows the extent of loss of cognate mRNA by quantitative reverse transcriptase PCR (qRT-PCR): levels of mRNA are shown four days after RNAi induction (Tet+) relative to the uninduced cells (Tet−), where qRT-PCR amplification has been set as 1.0 (values are the mean of four experimental repetitions and vertical lines denote standard deviation). <b>B</b> Representative images, showing aberrant cells with 0N1K DNA configuration seen 6 days after induction of RNAi of the gene indicated. Cells are shown with DNA stained by DAPI (N and K are arrowed), and as merge of DAPI and phase contrast images.