Effects of TbWee1 knockdown on the procyclic form of <i>T. brucei</i> cells.

<p>(A) Cells of strain 29-13 harboring the TbWee1-RNAi construct were incubated in culture medium with (+ Tet) or without (-Tet) 2.5 µg/ml tetracycline at 28°C. The cell growth rate was monitored daily, and the cell number was plotted in a logarithmic scale. The insets show the intracellular mRNA level after 3 days of RNAi as monitored by Northern blot. RNAr was used as loading control. Western blot of extracts of induced and non-induced cells were analyzed with anti-TbWee1 antibody (Right inset). (B) Time course of RNAi-induced <i>T. brucei</i> procyclic-form. Cells were stained with propidium iodide and subjected to FACS analysis to measure DNA content. The percentages of cells in G1, S and G2/M phases were determined with the ModFitLT software and plotted on the right panel.