Identification of transposon insertion <i>dGyk</i> hypomorph.

<p>Using glycerol hypersensitivity as a screen-able phenotype, we tested 6 fly lines with transposon insertions that map to the genomic loci for <i>dGyk</i> (e00237, 21039, and 22516) and <i>dGK</i> (f05001, 15351, and c06596). For each line, survival assays were performed using 7–10 day old male flies by placing the flies (n>100) on glycerol (1 M) and 1.3% agarose as food source. One fly line (<i>dGyk</i>^e00237 homozygous) was found to be glycerol hypersensitive (A). Heterozygous <i>dGyk</i>^e00237/TM6B flies were not glycerol hypersensitive. The 22516 and c06596 fly lines were homozygous lethal <i>i.e.</i> homozygous flies could not be assayed. Analysis of <i>dGyk</i>^e00237 homozygous 3^rd instar larvae revealed decreased <i>dGyk</i> expression (B), decreased GK activity (C), elevated glycerol (D), and normal triglyceride levels (E). For a control fly line, a transposon insertion line was used that had an identical type of P element (pBACRB) as used to create the <i>dGyk</i>^e00237 fly line. Survival analysis was performed using the log-rank test on the Kaplan and Meier data. Otherwise statistical analysis was performed using the Student's <i>t</i>-test. **<i>P</i><0.01, ***<i>P</i><0.001.