siRNA knockdown of uPA triggers incorporation of α-SMA into stress fibers.

<p>HCF were transfected with siRNA against uPA or control siRNA and after 24 hours (A) cells were immunostained for α-SMA. Bar = 10 um or 20 um. Images are from two independent experiments. (B) Metamorph analysis quantified changes in average cell size in each condition. ***p value<0.001. (C) Cell lysates were Western blotted for α-SMA and for uPA to confirm knock down. GAPDH indicates equal loading. Densitometry for α-SMA/GAPDH is shown. N = 3–5 for each experiment.