Knock down of MCM3AP protein by specific siRNAs.

<p>(A) HFFF cells were co-transfected with pCDNA3-GFP (GFP) or GFP-tagged MCM3AP (MCM3AP-GFP) together with an siRNA to MCM3AP (siMCM3AP) or a control siRNA (siCon) as indicated for 24 h. Transfected cells were analysed for GFP expression by immunofluorescence for GFP together with DNA counterstaining (Hoechst 33342) (B) HFFF cells transfected with GFP-tagged MCM3AP together with control siRNA (siCon), GANP siRNA (siGANP) or MCM3AP siRNAs (siMCM3AP) and analysed by western blotting with anti-GFP antibody to detect GFP-tagged MCM3AP expression. Actin loading controls are also shown (B). (C) Alternatively, cells were treated with siRNA (siCon) or GANP specific siRNA (siGANP) for 24 h, then analysed by western blot for GANP protein levels and actin (C).