Factors released by LPS and Stx1-treated ASTs increased HUVECd activation.

<p>HUVECd were stimulated with CM-Control, CM−LPS, CM−Stx1, CM−LPS+Stx1 or with 0.5 µg/ml de LPS (HUVECd+LPS). ICAM-1 (<b>A</b>) and E-selectin (<b>B</b>) expression was evaluated by FACS after 12 h or 3 h of stimulation, respectively. The release of von Willebrand Factor (vWF) was quantified by ELISA (<b>C</b>). <b>A</b>: ICAM-1 relative median fluorescence (RF) (Mean ± SEM, n = 4). *p<0.05 (LPS+Stx1 vs. Control); **p<0.001 (HUVEC+LPS vs. CM-Control, n = 3). <b>B</b>: E-selectin relative median fluorescence (RF) (Mean ± SEM, n = 4). **p<0.005 (CM−LPS+Stx1 vs. Control); *p<0.05 (CM−HUVEC+LPS vs. CM-Control, n = 4). Representative histograms of one independent experiment were plotted in the lower panels. <b>C</b>: Concentration (ng/ml) of vWF released (Mean ± SEM, n = 6). **p<0.01 (CM−LPS vs. CM-Control); **p<0.001 (CM−LPS+Stx1 vs. CM-Control); ^#p<0.05 (CM−LPS+Stx1 vs. CM−LPS).