Effect of atorvastatin on HMGB1 and IL-8 levels in HUVEC supernatants.

<p>4A – A time curve was built to evaluate HMGB1 release from HUVEC upon LPS stimulation. HMGB1 intensity in supernatants increases 8 hours after stimulation and a peak is observed at 24 hours with a decrease at 48 hours. 2B and C – HMGB1 and IL-8 levels are significantly higher at 24 hours in supernatants from HUVEC treated with LPS in comparison to unstimulated HUVEC while pre-incubation with 5 µM atorvastatin followed by LPS stimulation lowered HMGB1 and IL-8 levels significantly at 24 hours in HUVEC's supernatants. Experiments were performed twice and in duplicate. Data are presented as median and range, *<i>p</i><0.01 and **<i>p</i><0.001.