LT effects on cells expressing rNlrp1 constructs.

<p>(A) BMAJ cells (normally resistant to LT) that were retrovirally transfected with the indicated rNlrp1 constructs were treated with LT (PA+LF) for 10 h. Percent viability was assessed by MTT staining and calculated relative to untreated controls. Graph shown is from a single experiment, which is representative of eight identically performed experiments. Each point represents the average of three wells. Insets show staining of representative sensitive and resistant cell lines following toxin treatment. (B) Supernatant levels of IL-1β measured by ELISA. Cells were pretreated for 2 h with LPS (0.5–1 µg/ml), prior to LT treatment (7 h). Results shown are from a single experiment, which is representative of three similar experiments. Each point represents the average of duplicate wells in this experiment. (C) Protection of the sensitized BMAJ cell line transfected with rNlrp1fusion CDF53-LEW against challenge with LT (5 µg/ml) following treatment with 50 µM of each drug, or heat shock. Results are average of three experiments, with duplicate wells per treatment in each experiment. (D) Average supernatant IL-1β levels associated with protective treatments from (C) are shown. Results are the average of three experiments, with duplicate wells per treatment in each experiment.