Differential gene expression profile in intracellular versus extracellular bacteria.

<p><b>A</b>) Heat map of genes (n = 145) that displayed significantly (<i>P</i> : < 0.05) altered expression in intracellular versus extracellular <i>S. pyogenes</i> at 2h pi. RNA from 3 biological replicates were hybridized to microarrays and analyzed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035218#s4" target="_blank">material and method</a> section. Red color indicates up-regulation of genes; green down-regulation and black colored genes are not active as compared to house-keeping genes in the respective condition. <b>B</b>) Bar graph displaying differently expressed genes according to functional categories. Red bars indicate ≥75% of the genes are up-regulated, green bars indicate that ≥75% of the genes are down-regulated and black bars indicate that there is no consistent trend within the category.