Relative ZIP8 mRNA levels in <i>Slc39a8(</i>

<p><i>+</i><b><i>/</i></b><i>+</i><b><i>)</i></b><b>, </b><b><i>Slc39a8(</i></b><i>+</i><b><i>/neo)</i></b><b> and </b><b><i>Slc39a8(neo/neo)</i></b><b> genotypes.</b> (<b>A</b>) At three gestational ages, mRNA levels were determined in whole embryo/fetus, yolk sac, and placenta. (<b>B</b>) The mRNA was examined in PND1 liver, lung, kidney (<b>Kidn</b>), cerebrum (<b>Cereb</b>), cerebellum (<b>Cblm</b>), thymus (<b>Thym</b>), heart, and carcass (<b>Carc</b>). (“Carcass” is what remains after removal of liver, lungs, kidneys, heart, head and GI system.) Samples between five and 12 individual mice were examined. Mouse glyceraldehyde-3-phosphate dehydrogenase (<b>GAPDH</b>) was employed as the normalization control. The <i>Slc39a8(+/+)</i> wild-type ZIP8 mRNA/GAPDH mRNA ratio (left-most in each panel) and mRNA expression levels were expressed as linear fold-changes–relative to the normalized wild-type control. To lessen clutter in this figure or figure legend, all statistics are detailed in <b>Supplementary <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036055#pone.0036055.s011" target="_blank">Data S1</a></b> online.