Phenotypic characterization of specific CD8⁺ T cells of infected and/or AdASP-2 immunized A/Sn mice.

<p>A/Sn mice were infected or immunized as described in the legend of <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002699#ppat-1002699-g003" target="_blank">Figure 3A</a>. Splenic cells were collected nineteen days after infection/immunization. The histograms show FACS analysis on CD8⁺ cells (Gr. 1) and H-2K^k-TEWETGQI⁺ CD8⁺ cells (Gr. 2, 3, and 4) and the indicated marker (blue). Control cells were from naive mice (red lines). Results of CD44, KLRG1 and CD183 staining are presented as MFI and frequencies of the CD44^High, KLRG1^High or CD183^High cells, respectively. On the other hand, results of CD27 and CD62L staining are presented as MFI and frequencies of the CD27^Low or CD62L^Low cells. Representative analyses are shown from pools of cells from 3 mice. Stainings were performed 2 or more times with identical results.