An example demonstrating assembly coverage by mapping reads to a reference scaffold containing assembled sequences.

<p>The BWA tools mapped original 100 bp reads to the scaffold of all ABySS_55 sequences with criteria that the matches had > = 95% length and > = 90% identity. In this Figure, the top panel shows reference transcripts. The bottom panel shows coverage as the number of reads per nucleotide position in the reference genes. As shown in this example, many parts of the assembled sequences had coverage higher than 200.